Professional Bio for Daniel Pauletti  
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Daniel has recently expanded his technical skill base into SAS programming and has completed the Philadelphia University SAS training course for the pharmaceutical industry. He also received his SAS Base certification for SAS 9 and is working at a local CRO producing and validating analysis datasets, tables, listings, and figures for clinical trial data.

Previously he was engaged in drug discovery efforts at Cephalon, Inc. in West Chester, Pennsylvania. While there, he cloned, expressed, and purified many kinases in support of drug screening, crystallography studies, and protein/drug interactions. He also supported chemistry efforts by assaying compound degradation in liver microsomes using LC/MS techniques, as well as p450 gene regulation via RT-PCR methodology.

Prior to Cephalon, Daniel worked at Boehringer-Ingelheim Pharmaceuticals in Ridgefield, Connecticut, where he had a chance to apply his growing skills to research efforts in HIV. His main focus was to characterize the genetic changes occurring to HIV reverse transcriptase in patients undergoing concomitant drug therapy. After many years of work, the company announced that the FDA had approved Viramune (nevirapine), the first non-nucleoside inhibitor of HIV reverse transcriptase. The group Daniel was part of received several company awards for their work.

In 1984, Daniel met some scientists from Baylor who were going to establish a new department at the Southwest Foundation for Biomedical Research. He was offered an opportunity to join them in starting the Department of Virology and Immunology under the directorship of Dr. Gordon Dreesman. With a new threat from the HIV epidemic, the primate facility at SFBR would be an important resource. His duties there would be to utilize computer programs and immunoassays to predict the immunodominant epitopes on the envelope glycoprotein of HIV in the hope of developing a synthetic peptide vaccine. Some of the results were published in Science in 1986.

Daniel started his career shortly after receiving his bachelor's degree in the natural sciences from Indiana University of Pennsylvania in 1982. The first job he had was in the laboratory of Dr. James P. Chambers at the University of Texas Health Science Center in Houston, Texas. Dr. Chambers' interest in characterizing various isoforms of glucosidase allowed Daniel to begin learning how to apply all that stuff from college to the real world of scientific research.